FIELD: medicine.
SUBSTANCE: invention refers to microbiology and biotechnology. A method involves assessment of a prepared series of any live chicken cholera vaccine for harmlessness and immunogenicity from the perspective of repetition of a natural mechanism of micro- and microorganism interaction and a special role of environmental conditions. Birds of any species of body weight about 2 kg are vaccinated, and further they are infected on nasal and oropharyngeal mucosa at admissible optimal environmental temperature 20-22°C. A series of the live vaccine is considered to be harmless if all single vaccinated birds with twice standard dose are permanently clinically healthy, and to be immunogenic if a survival rate after infection with a lethal dose of a standardised control Pasteurella in a capsular version of a microbial cell is at least 8 (80 %) specifically resistant birds of the 10 twice vaccinated birds (every 7 days, in a standard dose, in 10 days after immunisation), and a death rate is at least 8 (80 %) amenable birds of the 10 not vaccinated (control) birds.
EFFECT: improved efficiency of the method.
7 dwg, 2 ex
