RECOMBINANT PLASMIDE DNA pCPBH FOR BIOSYNTHESIS OF POLYPEPTIDE WITH HUMAN CARBOXYPEPTIDASE B PROPERTIES AND RECOMBINANT STRAIN OF METHYLOTROPHIC YEASTS PICHIA PASTORIS - PRODUCENT OF POLYPEPTIDE WITH HUMAN CARBOXYPEPTIDASE B PROPERTIES Russian patent published in 2014 - IPC C12N15/63 C12N15/64 C12N15/66 C12N15/79 C12N1/16 

FIELD: chemistry.

SUBSTANCE: invention relates to the field of biotechnology. Claimed is a recombinant plasmid pCHBH for an expression of a sequence, coding procarboxypeptidase B in Pichia pastoris, which has a size of 10466 t.b.p. and consists of XhoI/EcoRI -fragment of DNA of vector pP1C9K with a size of 9246 t.b.p. and XhoI/EcoRI -fragment of DNA with a size of 1220 t.b.p., including a region, coding the signal peptide of α-factor of Saccharomyces cerevisiae, and a synthetic gene of human procarboxypeptidase B with a nucleotide sequence, corresponding to the sequence, represented on fig. 2. In addition, a recombinant strain of Pichia pastoris GS115CPBH - producent of human procarboxypeptidase, obtained as a result of the parent strain transformation by the said plasmid, is described.

EFFECT: invention makes it possible to obtain human procarboxypeptidase and a corresponding to it active form in an increased quantity, constituting not less than 9,0 units/ml in comparison with the prototype.

2 cl, 4 dwg, 4 ex