METHOD FOR ISOLATING DNA SODIUM SALT FROM ANIMAL TISSUE Russian patent published in 2007 - IPC A61K35/60 A61K35/48 

FIELD: medicinal and food industry.

SUBSTANCE: invention relates to a method for preparing biologically active substances. Proposed method for isolating DNA sodium salt from animal tissues involves defrosting raw at temperature +25 ± 5° in aseptic condition and extraction is carried out in reactor in 10% sodium chloride solution taken in the amount 3 l of solution per 1.5 ± 0.1 kg of raw at temperature +95-98°C at stirring and constant liquid volume. Filtration of extract is carried out at rate 0.5 l/min, not above, and precipitate containing DNA sodium salt is prepared by addition ethyl alcohol to extract in the amount 4 l per 1 kg of raw. Mixture is stirred and remained at temperature +2-10°C for 20-25 h. Supernatant liquid is decanted and precipitate is separated by centrifugation and dried. Method provides reducing labor intensity, simplifying technology and enhancing yield of the end product.

EFFECT: improved isolation and preparing method.

1 cl, 1 ex