FIELD: medicine, microbiology, preparative biochemistry, proteins.
SUBSTANCE: invention relates to vaccine preparations and to a method for preparing protective antigens based on secreting protein-containing fractions of microorganisms. Method involves culturing microorganisms on liquid nutrient media, separation of cultural medium from cells, purification of medium and concentrating by ultrafiltration with the removal threshold of protein of molecular mass 30 kDa and column chromatograpy by gel-filtration on Sephadex G-75 followed by purification on DEAE-Sepharose in gradient of concentration of NACl yielding fractions of molecular mass 30-35 kDa containing protein in the concentration 4-5 mg/ml. Method provides preparing immune preparation eliciting prophylactic and curative properties.
EFFECT: improved preparing method.
2 cl, 8 tbl, 5 dwg
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