FIELD: biotechnology.
SUBSTANCE: method provides culturing in the liquid culture medium of the virulent strain of bacteria Staphylococcus aureus No. 6 followed by separation of the culture medium from the bacteria by filtration through the filter PLASMAFILTER PLASMAFLUX PSu 2S with obtaining the filtrate. Ammonium sulphate to 80% saturation is added to the resulting filtrate to obtain the precipitate. The resulting precipitate is separated by centrifugation at 10000 g for 30 minutes and dissolved in phosphate buffer at pH 7.4 with subsequent microfiltration of the protein-containing fraction through the 0.22 mcm membrane and desalting on the column PD-10, purification and concentration by carrying out ion-exchange chromatography on the column of Q-sepharose, elution with 0.15 M NaCl and ultrafiltration on two filters of 100 and 30 kDa to obtain protein containing fraction with the molecular weight of 30-90 kDa and protein content of 0.5-1.0 mg/ml.
EFFECT: invention enables to obtain the products based on protective secreted protein-containing compound.
1 dwg, 5 tbl, 1 ex
