FIELD: chemistry.
SUBSTANCE: invention relates to the field of biotechnology. Claimed is a method of obtaining a high-titre antimicrobial serum. Method includes application of strain S.aureus No1991, characterised by immunogenicity ED50=(50-100)×106 microbial cells, weak virulence LD50=(0.5-2.0)×109 microbial cells; its inactivation and drying with dimethylketone by double processing with 3 volumes of dimethylketone. After that, immunisation of animals is carried out: two times subcutaneously and two times intravenously. In conclusion serum isolation is carried out.
EFFECT: invention can be used in medicine for estimation of antigen activity of anti-staphylococcal vaccines.
3 tbl
| Title | Year | Author | Number |
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| METHOD OF PRODUCING PROTECTIVE PROTEIN CONTAINING FRACTION OF BACTERIA | 2013 |
|
RU2533815C1 |
| POLYVALENT VACCINE FOR IMMUNOPROPHYLAXIS AND IMMUNOTHERAPY OF DISEASES CAUSED BY OPPORTUNISTIC MICROORGANISMS | 2002 |
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RU2209081C1 |
| METHOD OF OBTAINING PNEUMOCOCCAL HYPERIMMUNE SERA | 2016 |
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RU2624871C1 |
| STRAINS OF Streptococcus pneumoniae (VERSIONS) AND METHOD OF PRODUCING PROTECTIVE PROTEIN FRACTION, POSSESSING INTRASPECIFIC IMMUNOGENIC ACTIVITY | 2015 |
|
RU2601158C1 |
| TRAIN OF BACTERIA OCTAPHYLOCOCCUS ESED FOR PRODUCING STAPHYLLOCOCCUS DIAGNOSTICUM | 0 |
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SU1578190A1 |
| STRAIN "vFIRAVAX" FOR PRODUCING AN ATTENUATED ALIVE CULTURE VACCINE FOR PREVENTING VARICELLA | 2019 |
|
RU2693440C1 |
| RECOMBINANT PLASMID DNA pPA-OPRF-ETA CODING SYNTHESIS OF RECOMBINANT PROTEIN OPRF-ETA Pseudomonas aeruginosa, STRAIN Escherichia coli PA-OPRF-ETA PRODUCING RECOMBINANT PROTEIN OPRF-ETA Pseudomonas aeruginosa AND METHOD FOR PREPARING RECOMBINANT PROTEIN OPRF-ETA Pseudomonas aeruginosa | 2012 |
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RU2529359C2 |
| CELL-FREE ANTISTAPHYLOCOCCUS VACCINE FOR TREATMENT OF PATIENTS WITH CHRONIC STAPHYLOCOCCUS INFECTION | 1995 |
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RU2122862C1 |
| METHOD FOR PREPARING PROTECTIVE PROTEIN-CONTAINING FRACTION OF MICROORGANISM | 2006 |
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RU2311197C1 |
| RECOMBINANT PLASMID pPA-OPRFI DNA CODING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE, STRAIN Escherichia coli PA-OPRFI PRODUCING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE AND METHOD FOR PRODUCING HYBRID RECOMBINANT F-I PROTEIN OF Pseudomonas aeruginosa OUTER MEMBRANE | 2011 |
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RU2537006C2 |
