METHOD FOR PRODUCING MICROBIAL TRANSGLUTAMINASE Russian patent published in 2008 - IPC C12P21/00 C12N15/54 C12N15/55 C12N15/11

Abstract RU 2316594 C2

FIELD: biotechnology, microbiology, biochemistry, enzymes.

SUBSTANCE: method involves culturing corynebacterium comprising the inserted gene encoding neutral metalloprotease from Streptoverticillium and prostructural moiety of protransglutaminase is split off by neutral metalloprotease produced by this microorganism. Features of neutral metalloprotease are given in the invention text. Also, invention relates to nucleic acid molecule encoding neutral metalloprotease. Invention provides preparing protease that can be used for producing transglutaminase that splits prostructural moiety of protransglutaminase selectively.

EFFECT: improved producing method of enzyme.

8 cl, 12 dwg, 2 ex

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RU 2 316 594 C2

Authors

Umezava Jukiko

Jokojama Kejiti

Kikuti Josimi

Date Masajo

Onisi Norimasa

Dates

2008-02-10—Published

2004-03-05—Filed