WAY OF RECEPTION OF BIOMASS IN VITRO Russian patent published in 2008 - IPC A01H4/00 C12N5/04 

FIELD: processes.

SUBSTANCE: way contains preparation of explants, their sterilisation, placing of explants for callus formation and biomass growth in sterile test glasses with inoculated nutrient medium of Murasige-Skuga with addition of growth stimulants, thermostatting at 26±1°C. Sterilisation of explants carries out in distilled water by affecting of ultrasonic oscillations with intensity of 1...2 W / sq. sm. on working frequency 22...44 kHz during 3...6 minutes. At stages of callus formation and growth of biomass the test glasses with explants entrained on water medium and carry out through it affecting by ultrasonic oscillations with intensity not less than 10...15 W / sq. sm. on frequency 22...44 kHz. Affecting is spent from a bottom of test glasses with periodicity not less often than 1 time a day during not less than 6 minutes at each stage. The biomass gain makes 87.40 - 176.53%.

EFFECT: sterilisation efficiency and ability not infected explants to form callus increases.

5 tbl