POLYPEPTIDES TREATED WITH HAS, PARTICULARLY ERYTHROPOIETIN TREATED WITH HAS Russian patent published in 2008 - IPC C07K14/505 C07H1/06 C08B31/18 A61K47/36 

FIELD: chemistry.

SUBSTANCE: invention relates to the bio-organic chemistry and relates to the novel erythropoietin conjugate, the method of production and its pharmaceutical composition. The hydroxyethylamylum and erythropoietin conjugate containing one or several hydroxyethylamylum molecules, is invented. Each hydroxyethylamylum molecule is conjugated with erythropoietin via hydrocarbon fragment. The said hydroxyethylamylum has the molecular mass from 1 to 300 kDa and shows the correlation of the C₂:C₆-substitution in the range of 2-20 by hydroxylethyl groups. The method for producing the hydroxyethylamylum and erythropoietin conjugate is invented; in accordance with the method, the sialic acid is not necessarily removed, partially or totally, with use of enzyme and/or chemical way, and the erythropoietin end saccharide link is oxidised not necessarily partially or totally to receive the erythropoietin which is able to react with the modified hydroxyethylamylum. Into hydroxyethylamylum, the free hydrazide, hydroxylamine, thyol or semicarbazide functional group is entered to receive the modified hydroxyethylamylum which is conjugated with erythropoietin which is able to react with the modified hydroxyethylamylum, and the hydroxyethylamylum and erythropoietin conjugate containing one or several hydroxyethylamylum molecules, is obtained. The pharmaceutical composition contains the hydroxyethylamylum and erythropoietin conjugate with the erythropoietin activity, and the pharmaceutically acceptable solvent, adjuvant and/or carrier.

EFFECT: hydroxyethylamylum and erythropoietin conjugate with erythropoietin activity.

19 cl, 4 tbl, 25 dwg, 20 ex