FIELD: chemistry.
SUBSTANCE: invention relates to biochemistry. Disclosed is a method for synthesis of a conjugate of nona-β-(1→3)-glucoside with bovine serum albumin (BSA) using a squarate technique. Nona-β-(1→3)-glucoside is reacted with diethyl squarate first. The obtained ligand - monoamide of squaric acid - then reacts with BSA protein in a mixture of a borate buffer pH 9.0 and dimethyl sulphoxide. The ratio of the borate buffer to dimethyl sulphoxide is equal to 9:1.
EFFECT: method increases efficiency of the conjugation reaction and reduces ligand consumption by 20-25%.
5 ex
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