METHOD OF OBTAINING OF 6-O-α-D-GLUCOPYRANOSYLE-D-SORBITE Russian patent published in 2008 - IPC C12P19/12 C12P19/24 C12N9/04 C12N15/53 C07K16/40 C07K1/16 

FIELD: biology, biotechnologies.

SUBSTANCE: invention can be used in the food-processing industry. For obtaining of 6-O-αD-(1,6-GPS) isomaltulose would submit to the reactionary solution containing enzyme, possessing ability of catalyzing transformation of isomaltulose in 1,6-GPS from which allocate a target product after an incubation at temperature of 20-40°C. Before or during incubation in a reactionary solution add regenerative equivalents. Allocate enzyme with means of anion exchange and two affine chromatography from a crude extract of a microorganism of sort Gluconobacter containing a gene of enzyme, possessing ability of catalyzing transformation of isomaltulose in 1,6-GPS. The nucleic acid coding enzyme, possessing ability to catalyze transformation of isomaltulose in 1,6-GPS is obtained. The vector containing given nucleic acid, is intended for provision of an expression of this enzyme in a host cell.

EFFECT: invention application allows obtaining 6-O-αD-D-D-sorbite from isomaltulose by means of the unique enzymatic reaction.

26 cl, 2 dwg, 1 tbl, 4 ex