FIELD: medicine; cellular technologies.
SUBSTANCE: method of production of skin cell culture includes skin explants sampling and placing of fragments thereof into Petri dish, filling with culture medium, followed by cultivation and removal of produced culture. Herewith explant pieces are fixed, pressing it to Petri dish surface with glass plate inserted in brace of Petri dish. Pressing plate area is 40-70% of Petri dish area, and number of fixation points (plate angles) is at least three, with plate thickness at least 0.5 mm. Grown cell culture is collected not only from Petri dish surface, and also from pressing plate surface. Invention allows for produced skin cell culture in great volume for short time period.
EFFECT: reliable fixation of explants fragments, higher efficiency and acceleration of skin call cultivation.
1 ex, 1 dwg
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