METHOD OF BIOLOGICAL ACTIVE ADDITIVE PRODUCTION Russian patent published in 2009 - IPC A23L1/30 A23J3/00 A61K35/74 C12N1/14 

FIELD: food products.

SUBSTANCE: method of biological active additive production includes three-staged processing of yeast cells water suspension with exogenous ferments to obtain target product in the form of fermentolisate. During the first and the second stage the initial and secondary zymogene compositions are used. The initial zymogene composition contains for 1 g of dried yeast 0.05-5.0 activity of proteinase and 10.0-50.0 activity units of mix of endo-beta-gluconase and exo-beta-gluconase. The secondary zymogene composition contains 50.0-300.0 activity units of mix of endo-beta-gluconase and exo-beta-gluconase, 5.0-20.0 activity units of mannanase and 0.01-0.5 activity units of chitinase. The processing of yeast cells suspension of the initial zymogene composition is performed at 54-56°C during 1 hour 45 min. - 2 hours 15 min. The processing of the secondary zymogene composition obtained after the first stage is performed at 45-48°C during 3 hour 45 min. - 4 hours 15 min. During the third stage processing of the obtained mass is performed with ferments of the secondary zymogene composition at temperature of 35-38°C during 6-12 hours. The product output equals 92%, the concentration of amine nitrogen is 6.3%.

EFFECT: obtaining of target product with high output and high aminoacid content.

8 cl, 3 ex