FIELD: medicine.
SUBSTANCE: invention refers to physiology and pharmaceutics. The method is ensured by preparing convoluted brain section with registering initial and following subsequent parametres after the sections are incubated in pentylenetetrazol medium. Effect is evaluated in comparing the initial and following parametres. Therefor the prepared sections are placed in pentylenetetrazol (chemoconvulsant) in epileptogenic concentration 1-10 mkg/ml, dissolved in incubatory medium in volume 1 ml, and kept for 15 minutes. Then pentylenetetrazol effect is stopped and followed by effect of hsp-70 in concentration 1 mg/ml within 30 minutes. Thereafter the sections are washed with physiologic saline with registering the caused bioelectric activity of cells. Presence or absence of epileptic categories shows effect of hsp-70 on nervous cell integrity in simulated epilepsy.
EFFECT: evaluated activity of specified set of protein hsp-70.
2 tbl, 1 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF HSP-70 ANALYSIS FOR ACTION ON CEREBRAL TISSUE | 2007 |
|
RU2361221C2 |
| ESTIMATE METHOD FOR PROTEIN ACTION ON BRAIN TISSUE | 2007 |
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| METHOD OF INVESTIGATION OF PROPERTIES OF HEAT SHOCK PROTEIN (HSP70) | 2007 |
|
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| WAY OF DETERMINATION OF INFLUENCE OF PROTEIN ON BRAIN TISSUE | 2007 |
|
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| PHARMACEUTICAL COMPOSITION FOR PREVENTING AND TREATING PAROXYSMAL CONDITIONS | 2011 |
|
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| INTERLEUKIN-10, USING IT AND BASED COMPOSITION FOR TREATING BRAIN NEURODEGENERATIVE PROCESSES | 2012 |
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| METHOD OF SIMULATION AND RESEARCH OF CONSEQUENCES OF HEMORRHAGIC STROKE | 2005 |
|
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| 4-BENZOYLPYRIDINE OXIME DERIVATIVES HAVING ANTICONVULSANT ACTIVITY AS AGENTS FOR TREATMENT OF EPILEPSY AND PAROXYSMAL STATES | 2016 |
|
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| ESTIMATE METHOD OF BIOLOGICALLY ACTIVE SUBSTANCE (BAS) IMPACT ON BRAIN TISSUE | 2007 |
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| METHOD OF SIMULATION AND EXAMINATION OF HEMORRHAGIC STROKE CONSEQUENCES | 2007 |
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