METHOD OF HSP-70 ANALYSIS FOR ACTION ON CEREBRAL TISSUE Russian patent published in 2009 - IPC G01N33/68 G01N33/483 

FIELD: medicine.

SUBSTANCE: invention refers to physiology and pharmaceutics. The method is ensured by preparing survival brain section with registering the initial and following parametres after incubation of the sections cuts in the medium with the analysed protein to determine its action as compared to the initial parametres and those after protein action. The method involves registration of initial bioelectric activity of the section cells and incubation of the sections in the protein medium concentrated 1 mg/ml within 20 minutes. Then protein action is stopped, while the sections are exposed to pentylenetetrazol (chemoconvulsant) in epileptogenic concentration 1-10 mkg/ml within 40 minutes. Then the sections are washed with physiologic saline, and bioelectric activity of cells is registered. The absence or presence of epileptic categories shows action of HSP-70 on protection of nervous cells in simulated epilepsy in experiment.

EFFECT: possibility for analysis HSP-70 of specific set for its activity.

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