METHOD FOR PREPARING FINE-DISPERSED MYCOBACTERIA CELL CULTURE SENSITIVE TO MYCOBACTERIOPHAGE INFECTION Russian patent published in 2009 - IPC C12N1/20 C12Q1/04 

FIELD: medicine.

SUBSTANCE: method for preparing fine-dispersed mycobacteria cell cultures sensitive to mycobacteriophage infection involves primary cultivation of mycobacteria in a nutrient medium with nonionic detergent being either Tween 80, or Tween 20, or Span 85 in amount 0.25-0.5 wt %, and/or ionogenic detergent being sodium lauroyl sarcosinate within 0.01-0.03 wt %. After one or more reinoculation, cultivation is carried out without detergent. Herewith throughout the entire cycle, mycobacteria cell cultivation is enabled in the nutrient medium containing, g/l: trypton - 15, yeastrel - 5, glucose - 40, sodium chloride - 2.5, potassium hydrophosphate - 5, calcium chloride - 0.111.

EFFECT: higher dose accuracy of fine-dispersed cell mass combined with bacteriophage infection of large quantity of sampled cell mass with reduced work content of the process.

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