RECOMBINANT PLASMID DNA, CONTAINING GENES OF BUTANOL SYNTHESIS FROM Clostridium acetobutylicum (VERSIONS), RECOMBINANT STRAIN Lactobacillus brevis - PRODUCER OF N-BUTANOL (VERSIONS) AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF N-BUTANOL Russian patent published in 2009 - IPC C12N15/74 C12N1/21 

FIELD: biotechnologies.

SUBSTANCE: invention is related to biotechnology, in particular to production of n-butanol by means of carbohydrate-containing raw material fermentation with recombinant bacteria. The following plasmid DNA are constructed: pBCS, containing genes of butanol biosynthesis crt, bed, etfB, etfA and hbd from C.acetobutylicum and pTHL-BCS, containing, apart from earlier mentioned, gene thi from C.acetobutylicum. Plasmids may be replicated both in gram-negative (E.coli) and in gram-positive (L.brevis) bacteria. Recombinant strain-producers of n-butanol are produced on the basis of bacteria Lactobacillus brevis: strain Lactobacillus brevis VKPM V-10044, containing plasmid pBCS; strain Lactobacillus brevis VKPM V-10043, containing plasmid pTHL-BCS, which are able to synthesise butanol and resistant to its concentration of 2.0-2.8 wt % in liquid medium. Method is developed for synthesis of butanol on the basis of recombinant bacteria L.brevis, which combine ability to synthesise butanol with resistance to its concentrations in liquid medium of at least 2.0 wt %, which makes it possible to produce butanol using both glucose and xylose as source of carbon in mediums for cultivation.

EFFECT: invention makes it possible to increase synthesis efficiency.

5 cl, 2 tbl, 8 ex