FIELD: medicine.
SUBSTANCE: method consists in cultivation of the microorganism Clostridia acetobutylicum with the butirate kinase coding gene buk being deleted in the appropriate culture medium containing a carbon source, and n-butanol extraction from the culture medium. The microorganism used in the method can also contain inactivated mutations in endogenous genes coding a polypeptide with lactate dehydrogenase activity, a polypeptide with phosphotransacetylase or lactate kinase activity and have attenuation in the gene coding a polypeptide with hydrogenase activity.
EFFECT: invention provides preparing high n-butanol yield from the inexpensive carbon substrate, such as glucose or other sugars under action of the genetically stable Clostridia acetobutylicum.
10 cl, 7 tbl, 8 ex
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