PROCESS AND APPARATUS FOR ISOLATING PROTEIN OF INTEREST IN HETEROGENEOUS TISSUE CULTURE LIQUID MIXTURE AND PURIFICATION THEREOF Russian patent published in 2010 - IPC C07K14/47 C07K14/435 C12P21/06 B01D61/14 

FIELD: chemistry; biochemistry.

SUBSTANCE: present invention relates to a process and apparatus for isolating and purifying protein of interest in a stream of a tissue culture liquid obtained from a continuous perfusion fermentation process. The proposed apparatus, in which sterile conditions are maintained, includes a continuous perfusion fermentation system, a continuous particle removal system integrated with the perfusion fermentation system and adapted for continuous reception of tissue culture liquid therefrom and continuous production of clarified tissue culture liquid, and a continuous purification system integrated with the particle removal system and adapted for continuous reception clarified tissue culture liquid therefrom and constant production of the extracted product which contain the protein of interest, where the continuous purification system is an ultrafiltration system. The process involves obtaining heterogeneous tissue culture liquid mixture containing the protein of interest during a continuous perfusion fermentation process, continuous removal of large particle impurities from the liquid mixture to obtain clarified tissue culture liquid containing protein of interest, and purification of the protein from the clarified culture liquid through ultrafiltration. Specific flow rate of the tissue culture liquid mixture during continuous perfusion fermentation, continuous removal of impurities and continuous purification is kept constant.

EFFECT: design of an efficient method of isolating and purifying protein.

9 cl, 17 dwg, 2 tbl