FIELD: medicine.
SUBSTANCE: DNA is recovered from each germ in a parental and hybrid soya seed group. The recovered DNA is amplified by the polymerase chain reaction. The amplification is enabled by microsatellite SATT1, SATT9 and SOYPR 1 loci with using primers flanking these loci. The amplification products are electrophoretically separated in the coloured agarose gel.
EFFECT: comparison of electrophoretic spectra of each germ and spectra of its parental forms in ultraviolet rays allows recovering hybrid germs by observing two fractions on an electrophoregram matched with the fractions of parental forms.
2 cl, 3 dwg, 1 ex
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