FIELD: biotechnology.
SUBSTANCE: method envisages preparation of a reaction mixture containing a buffer solution and DNA analyzed sample. Methyltransferase with activity of 2 units/mcl is added to the mixture of DNA, modifying cytosine in C5 position selected from: Fsp4HI, HaeIII, HspAI, AluI, depending on the site that is to be split, mixture is incubated for one hour, obtained C5-methylated DNA is processed by MD-endonuclease of ElmI with activity of 2 units/mcl or PkrI with activity of 2 units/mcl depending in which position is necessary to hydrolyse DNA.
EFFECT: using this method it is possible to perform exhaustive site specific DNA hydrolysis at six-and eight-nucleotide sequence, non-decomposable by traditionally used by restriction endonucleases.
3 cl, 3 dwg, 6 ex
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