FIELD: chemistry; biochemistry.
SUBSTANCE: invention relates to biotechnology and is a method of producing purine nucleosides using a bacterium belonging to the Bacillus genus, containing a fragment of DNA which codes mutant bacterial phosphoribosyl pyrophosphate synthetase, in which the L-amino acid residue which corresponds to position 120, 135 or 194 in the wild type phosphoribosyl pyrophosphate synthetase sequence from Bacillus subtilis is substituted with a residue of another L-amino acid, and sensitivity of the said enzyme to inhibiting purine nucleotides on the type of feedback is low.
EFFECT: invention enables to obtain highly effective purine nucleosides.
13 cl, 2 dwg, 2 tbl, 5 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF PRODUCING PURINE RIBONUCLEOSIDE AND RIBONUCLEOTIDES | 2008 |
|
RU2422510C2 |
| YdhL PROTEIN FROM Bacillus amyloliquefaciens, DNA FRAGMENT, BACTERIUM BELONGING TO GENUS Esherichia OR Bacillus AS PRODUCER OF PURINE NUCLEOSIDES, METHOD FOR PRODUCTION OF PURINE NUCLEOSIDES AND PURINE NUCLEOTIDES | 2005 |
|
RU2294962C2 |
| METHOD OF PURINE NUCLEOZIDES AND NUCLEOTIDES PRODUCTION BY FERMENTATION WITH APPLICATION OF BACTERIA BELONGING TO GENUS Escherichia OR Bacillus | 2006 |
|
RU2365622C2 |
| METHOD FOR PREPARING PURINE NUCLEOSIDE AND NUCLEOTIDE, STRAIN AS PRODUCER OF PURINE NUCLEOSIDE (VARIANTS) | 2002 |
|
RU2239656C2 |
| METHOD FOR PRODUCTION OF INOSIBE AND INOSINE-5'-MONOPHOSPHATE, BACTERIUM STRAIN OF GENUS BACILLUS AS INOSINE PRODUCER (VARIANTS) | 2002 |
|
RU2260040C2 |
| METHOD FOR MICROBIOLOGIAL SYNTHESIS OF PURINE NUCLEOSIDE OF 5'-AMINOIMIDAZOLE-4-CARBOXAMIDE RIBOSIDE (AICAR) AND BACILLUS SUBTILIS STRAIN - PRODUCER OF AICAR | 2008 |
|
RU2405833C2 |
| METHOD FOR PRODUCING PURINE NUCLEOSIDES BY FERMENTATION METHOD USING MICROORGANISMS BELONGING TO BACILLUS GENUS | 2005 |
|
RU2320717C2 |
| MUTANT PHOSPHORIBOZYL PYROPHOSPHATE SYNTHETASE, DNA FRAGMENT, BACTERIA OF GENUS ESCHERICHIA AS L-HISTIDINE AND METHOD FOR PRODUCTION OF L-HISTIDINE | 2004 |
|
RU2282660C2 |
| NEW ADENYL-SUCCINATE SYNTHETASE AND METHOD FOR PRODUCING PURINE NUCLEOTIDES USING IT | 2018 |
|
RU2770464C1 |
| BACTERIA Bacillus subtilis PRODUCING 5`-AMINOIMIDAZOLE-4-CARBOXAMIDERIBOSIDE (AICAR), AND METHOD OF MICROBIOLOGICAL SYNTHESIS OF AICAR BY CULTURING THIS BACTERIUM | 2013 |
|
RU2542387C1 |
