METHOD OF NEURONAL CELL DIFFERENTIATION FROM EMBRYO STEM (ES) Russian patent published in 2010 - IPC C12N5/00 C12N5/07 C12N5/71 

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology, particularly to a method of induced differentiation of embryo stem (ES) cells in neuronal precursor cells. The presented method involves ES cell culture by sowing the ES cells of density approximately 0.5 × 10⁵ - 2 × 10⁵ cells in cm² and dissociating the ES cells 2 days after sowing. Then, cell aggregates (CA) are formed that involves sampling the cells of high proliferative activity of doubling time within 0 to 24 hours and sowing these cells of density approximately 0.5 × 10⁵ - 5 × 10⁵ cells in ml to form the CAs. Further, the cell aggregates are processed with retinoic acid (RA). Then, the CAs are dissociated to prepare a neuronal precursor cell culture.

EFFECT: presented invention allows preparing substantially homogeneous neuron population wherein practically all neurons belong to the same certain neuronal cell differentiation line, to the same phenotype, cell type and to the same differentiation stage.

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