FIELD: biotechnology.
SUBSTANCE: disclosed is a method of producing dopaminergic neurons by directed differentiation of induced human pluripotent stem cells (IHSPC) in vitro. Method is realized for 38 days and involves a reduced number of changes of compositions of culture media to three.
EFFECT: invention enables to expand the range of technical means for producing dopaminergic neurons from IHSPC using directed differentiation in vitro, and the present invention can be used to produce a biomedical cell product for treating Parkinson's disease.
1 cl, 8 dwg, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| EVALUATION METHOD OF MORPHOFUNCTIONAL STATE OF INDUCED PLURIPOTENT STEM CELLS OF PARKINSONIAN PATIENTS, WHICH ARE DIFFERENTIATED TO DOPAMINERGIC NEURONS | 2012 |
|
RU2501853C1 |
| NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES RECOVERED FROM NEURONAL PROGENITOR CELLS UNDER HYPOXIC CONDITIONS, AND A METHOD FOR PRODUCTION THEREOF | 2019 |
|
RU2732600C1 |
| NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES ISOLATED FROM NEURONAL PROGENITOR CELLS UNDER HEAT SHOCK AND METHOD FOR PRODUCTION THEREOF | 2019 |
|
RU2752906C2 |
| NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES ISOLATED FROM GLIAL PROGENITOR CELLS UNDER CONDITIONS OF HEAT SHOCK, AND A METHOD FOR PRODUCTION THEREOF | 2019 |
|
RU2732599C1 |
| CONVERSION OF SOMATIC CELLS INTO INDUCED REPROGRAMMED NEURAL STEM CELLS (IRNSC) | 2011 |
|
RU2562111C2 |
| METHOD FOR OBTAINING BIOMEDICAL CELL PRODUCT FOR TREATMENT OF ONCOLOGICAL, NEURODEGENERATIVE AND AUTOIMMUNE DISEASES | 2019 |
|
RU2774350C2 |
| NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES ISOLATED FROM NEURONS AND GLIAL CELLS OBTAINED BY DIRECTED DIFFERENTIATION OF INDUCED HUMAN PLURIPOTENT STEM CELLS | 2018 |
|
RU2690846C1 |
| PRODUCTION OF TERMINAL-DIFFERENTIATED DOFAMINNERGIC NEURONES FROM EMBRIONIC HUMAN FOUNDER CELLS | 2004 |
|
RU2345133C2 |
| METHOD FOR PREPARING A NOOTROPIC COMPOSITION BASED ON POLYPEPTIDE COMPLEXES ISOLATED FROM NEURONS AND GLIAL CELLS OBTAINED BY DIRECTIONAL DIFFERENTIATION OF INDUCED HUMAN PLURIPOTENT STEM CELLS | 2018 |
|
RU2690498C1 |
| METHOD FOR ASSESSING NEUROPROTECTIVE PROPERTIES OF VITRO SUBSTANCES AND THE TEST SYSTEM FOR ITS IMPLEMENTATION | 2016 |
|
RU2646446C1 |
