FIELD: biotechnologies.
SUBSTANCE: inventions represent synthetic oligonucleotide primers: P1 - 5'-TGGTTACTATTCCATCACCATT-3' (annealing site 11-32 base pairs), P2 - 5'-CGAAACGTCACTTTCGCAAC-3' (annealing site 259-278 base pairs) and method for detection of DNA of infectious anaemia virus of chickens with their help. The method consists in the fact that primers flank the section of the virus genome, which includes CpG islands and VNTR repetitions in the polymerase chain reaction in real time mode. In case of positive reaction, a peak of the melting curve 92C° appears, and when the reaction is confirmed by means of electrophoresis a fragment is visualised in the gel that corresponds to the size of 268 base pairs.
EFFECT: method of diagnostics makes it possible to determine quantitative content of a virus in tissues and may be used to diagnose infectitious anaemia of chickens.
2 cl, 3 dwg, 4 tbl, 5 ex
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