FIELD: medicine.
SUBSTANCE: sequence typing is used to differentiate Yersinia pestis strains. The technique provides recovery of chromosomal DNA of the investigated strain, polymerase chain reaction (PCR) with amplification of rhaS, araC, metB, asp A and thiH gene fragments to be analysed for nucleotide sequences. A genotype of the investigated strain is stated by nucleotides being in the positions 482, 494, 671 reHarhaS, in the position 773 of the gene rhaS, in the positions 988 and 989 of the gene metB, in the positions 1087-1089 of the gene aspA and in the position 552 of the gene thiH. A sequence type (ST) of the Y. pestis strain is specified by rhaS, araC, metB, aspA and thiH gene alleles, while the subspecies differentiation is enabled by comparing to the sequence types of the major and minor subspecies. The sequence type (ST) is specified for each subspecies.
EFFECT: use of the method provides fast, reliable and effective differentiation of Yersinia pestis subspecies.
2 tbl, 5 ex
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| METHOD FOR INDICATION AND IDENTIFICATION OF STRAINS OF THE PLAGUE PATHOGEN BY THEIR BELONGING TO THE SPECIES YERSINIA PESTIS, TO SUBSPECIES, BIOVARS, PHYLOGENETIC BRANCHES AND BY THE PRESENCE OF GENES OF PATHOGENICITY MAIN FACTORS BY THE DNA-CHIP METHOD | 2020 |
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