FIELD: biotechnologies.
SUBSTANCE: method provides extraction of chromosomal DNA of the studied strain, amplification of DNAs of targets in polimerase chain reaction using constructed primers, sequencing of amplificated fragments. Genovariation of the studied strain of plague agent is identified by variable nucleotides, marker for separate genovariations.
EFFECT: method allows to identify genovariations of the named strains in efficient and reliable way.
1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF DETERMINING THE PHYLOGENETIC AFFILIATION OF STRAINS OF YERSINIA PESTIS OF THE MAIN SUBSPECIES BY ALLELE-SPECIFIC PCR IN REAL TIME | 2022 |
|
RU2799415C1 |
| DIFFERENTIATION METHOD OF BIOVARS AND GENOVARIATIONS OF Yersinia pestis STRAINS OF MAIN SUBSPECIES BY MEANS OF POLYMERASE CHAIN REACTION | 2014 |
|
RU2565554C2 |
| METHOD FOR INDICATION AND IDENTIFICATION OF STRAINS OF THE PLAGUE PATHOGEN BY THEIR BELONGING TO THE SPECIES YERSINIA PESTIS, TO SUBSPECIES, BIOVARS, PHYLOGENETIC BRANCHES AND BY THE PRESENCE OF GENES OF PATHOGENICITY MAIN FACTORS BY THE DNA-CHIP METHOD | 2020 |
|
RU2734636C1 |
| METHOD FOR IDENTIFICATION OF YERSINIA PESTIS STRAINS OF A MEDIEVAL BIOVAR WITH SUBSEQUENT DIFFERENTIATION BY PHYLOGENETIC AFFILIATION BY POLYMERASE CHAIN REACTION WITH HYBRIDIZATION-FLUORESCENT ACCOUNT OF RESULTS | 2018 |
|
RU2705813C1 |
| METHOD FOR IDENTIFYING YERSINIA PESTIS AND YERSINIA PSEUDOTUBERCULOSIS AND SIMULTANEOUS DIFFERENTIATION OF YERSINIA PESTIS OF MAIN AND CENTRAL ASIAN SUBSPECIES BY MULTIPLEX PCR | 2020 |
|
RU2737775C1 |
| METHOD OF SUBSPECIES DIFFERENTIATION OF PLAGUE AGENT STRAINS USING POLYMERASE CHAIN REACTION METHOD | 2014 |
|
RU2552611C2 |
| METHOD TO DIFFERENTIATE STRAINS OF PLAGUE AGENT OF PRIMARY SUBTYPE OF MEDIEVAL AND ANTIQUE BIOVARS BY METHOD OF POLYMERASE CHAIN REACTION | 2012 |
|
RU2496882C2 |
| METHOD OF SUBSPECIFIC DIFFERENTIATION OF Yersinia pestis STRAINS BY MULTILOCUS SEQUENCE TYPING | 2009 |
|
RU2415948C1 |
| METHOD FOR DIFFERENTIATING YERSINIA PESTIS STRAINS OF VARIOUS SUBSPECIES AND BIOVARS BY POLYMERASE CHAIN REACTION AND MULTILOCUS SEQUENCE TYPING | 2011 |
|
RU2471872C1 |
| METHOD OF SUBSPECIFIC DIFFERENTIATION OF PLAGUE CAUSATIVE AGENT STRAINS BY SEQUENCING METHOD | 2009 |
|
RU2404256C1 |
