FIELD: medicine.
SUBSTANCE: tissue sample must be fixed and placed in paraffin, cuts are made, deparaffinated, washed in tris-buffer, incubated with reagent and processed with developing agent. Difference of method lies in the following: as reagent used are antibodies to VEGFR1 in dilution 1:50, duration of incubation with reagent in wet chamber at temperature 25°C constitutes 60 minutes.
EFFECT: method makes it possible to stain mast cells on histological preparation in a differentiated way.
5 dwg, 1 ex
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