RECOMBINANT BACTERIAL PLASMID EXPRESSING PATHOGEN PROTEIN AND BIOLOGICALLY ACTIVE FRAGMENT OF ORNITHINE DECARBOXYLASE IN MAMMAL CELLS FOR DEVELOPING AGENT FOR PREVENTION OF VIRAL INFECTIOUS DISEASES (BY EXAMPLE OF TICK-BORNE ENCEPHALITIS VIRUS) Russian patent published in 2011 - IPC C12N15/00 

FIELD: medicine.

SUBSTANCE: method of DNA-vaccine preparation modification is based on fusion of a non-structural protein NS1 TEV gene with a fragment of a mouse ornithine decarboxylase protein gene. The non-structural protein NS1 TEV gene is cloned in a plasmid used for protein expression in eukaryote cells. It is followed by site-directed mutagenesis of a 5'-terminal fragment of the viral gene for removing a terminating codon. The fragment of the ornithine decarboxylase gene coding 53 S-terminal amino acids and containing the terminating codon on the 5'-end is cloned. The plasmids are developed in E.coli cells and purified in the low endotoxin environment. It is followed by transfection by a plasmid preparation of eukaryote cell culture with immunoblot analysis of chimeric protein synthesis in cells. Mice are immunised to study the protective properties of the modified preparation.

EFFECT: invention allows higher effectiveness of the DNA-vaccine preparations, as the agents for prevention of development of tick-borne encephalitis.

5 dwg, 1 ex