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2 280 691

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IPC

C12N15/70(2006-01-01)

C12N15/33(2006-01-01)

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Application

2004130387/13, 2004-10-19

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Start date

2004-10-19

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Actual filing date

2004-10-19

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Published

2006-07-27

(72)

Inventor

Markov Viktor IvanovichKovtun Anatolij LeonidovichMakhlaj Aleksandr AleksandrovichMerkulov Vadim Anatol'EvichKarpov Vadim L'VovichMel'Nikova Ekaterina Valer'EvnaMaksimov Vladimir Alekseevich

(73)

Holder

Virusologicheskij Tsentr Nii Mikrobiologii Ministerstva Oborony Rossijskoj Federatsii

RECOMBINANT PLASMID pCI-neo-ODC-nsP1 AND ITS VARIANTS pCI-neo-ODC-E2-L AND pCI-neo-ODC-E2-M ENCODING VENEZUELAN EQUINE ENCEPHALOMYELITIS (VEE) VIRUS PROTEINS AND ORNITHINE DECARBOXYLASE PROTEIN DESIGNATED FOR DEVELOPMENT OF AGENTS FOR PROPHYLAXIS OF VIRAL INFECTIOUS DISEASES (WITH VEE VIRUS EXAMPLE) Russian patent published in 2006 - IPC C12N15/70 C12N15/33

Abstract RU 2280691 C2

FIELD: genetic engineering, biochemistry, virology.

SUBSTANCE: invention proposes recombinant plasmid pCI-neo-ODC-nsP1 and its variants pCI-neo-ODC-E2-L and pCI-neo-ODC-E2-M encoding proteins of Venezuelan equine encephalomyelitis (VEE) and ornithine decarboxylase protein. Plasmids are prepared by the sequential cloning gene-equivalent of non-structural protein nsP1 (fragments of L,M of the structural protein E2) of VEE virus as component of gene encoding enzyme ornithine decarboxylase being firstly into prokaryotic plasmid vector pET-23b and then into eucaryotic plasmid vector pCI-neo. Prepared plasmids can be used in the development of protective preparations used against Venezuelan equine encephalomyelitis virus as a prophylactic agent with respect of this pathogen.

EFFECT: valuable biological and medicinal properties of plasmid and agents.

3 cl, 2 tbl, 6 dwg

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RU 2 280 691 C2

Authors

Markov Viktor Ivanovich

Kovtun Anatolij Leonidovich

Makhlaj Aleksandr Aleksandrovich

Merkulov Vadim Anatol'Evich

Karpov Vadim L'Vovich

Mel'Nikova Ekaterina Valer'Evna

Maksimov Vladimir Alekseevich

Dates

2006-07-27—Published

2004-10-19—Filed