METHOD FOR PRESENTATION OF VIRAL ANTIGEN IN IMMUNE SYSTEM OF HOST BASED ON PROTEASOME-MEDIATED TECHNOLOGY DESIGNATED FOR DEVELOPMENT OF METHODS OF BIOLOGICAL PROTECTION Russian patent published in 2006 - IPC A61K48/00 A61K39/12 C12N15/52 A61P31/12 

FIELD: immunology, molecular biology.

SUBSTANCE: invention relates to a method for presentation of viral antigens of the host immune system based on proteasome-mediated technology designated for development of methods of biological protection. Method involves using recombinant plasmids pCI-neo-ODS-nsP1, pCI-neo-ODS-E2-L and pCI-neo-ODS-E2-M. Recombinant plasmids pCI-neo-ODS-nsP1, pCI-neo-ODS-E2-L and pCI-neo-ODS-E2-M are prepared by the successive cloning gene-equivalents of nonstructural protein nsP1, L and M fragments of structural protein E2 of virus VEL, respectively, as components of gene encoding enzyme ornitine decarboxylase being firstly into prokaryotic plasmid vector pET-23b and then into eucaryotic plasmid vector pCI-neo. The advantage of invention involves the development of polynucleotide vaccines inducing the cell-mediated immune response. The proposed invention represents the firstly developed in Russian Federation method of DNA vaccination based on using proteasome-mediated technology as result of molecular-biological and immunological investigations.

EFFECT: improved method for presentation of antigens.

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