FIELD: medicine.
SUBSTANCE: invention refers to genetic engineering, tissue technologies and medicine. The method for producing the mesenchymal stem cells of pluripotent lines of the human stem cells involves producing the embryoid bodies of the human pluripotent stem cells, attaching the embryoid bodies to a Petri dish to induce spontaneous differentiation of the embryoid bodies into the mesenchymal stem cells, culturing and proliferating the mesenchymal stem cells with maintaining the identity of the mesenchymal stem cells, wherein the induction of spontaneous differentiation at the stage is performed by looping an autologous cytokine with no outer cytokine added; there are also produced the respective cells, using them, a kit and a culture method.
EFFECT: invention can be used for producing the mesenchymal stem cells in regenerative medicine and cell therapy.
15 cl, 11 dwg, 3 ex
