FIELD: biotechnologies.
SUBSTANCE: there proposed is the method for obtaining endotheliocytes from human embryonic stem cells, which involves differentiation of human embryonic stem cells on the substrate and in synthetic medium. Method allows obtaining endotheliocytes with high efficiency, choosing pure population of functional endotheliocytes that gained the same epigenetic status of key factors of transcription and homeostasis of genes sustaining as their analogues did, which are differentiated in embryo in vivo.
EFFECT: obtained endotheliocytes can be widely used in medical practice.
2 cl, 11 dwg, 4 tbl, 5 ex
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