METHOD OF OBTAINING CARDIOMYOCYTAL MATRIX Russian patent published in 2011 - IPC C12N5/73 

FIELD: medicine.

SUBSTANCE: method includes increase of biomass of human germ stem cells of non-feeder line hESKM-05 with application of main medium mTeSR in flasks covered with matrigel. After that, performed is subcultivation by means of 0.05% dispase solution. Then, in order to form germ stem cells of embrioid bodies, by means of dispase cells are transferred into "коДМЕМ" medium with addition of 10% substituent of SR serum, 100 mcg/ml of sulfate canamicine, 1mM of L-glutamine solution and 1 mM solution of essential amino acids, as well as 2 mcg/ml solution of 5-asa-2-seoxycitidine or 2 mcM of sodium butirate solution and stimulated for 3 days. After that retransmission onto prepared collagen-chitosane matrix and cultivation in nutritional medium "коДМЕМ" with addition of 1 mM solution of essential amino acids, 1 mM of L-glutamine, 10% substituent of SR serum, 10^-7 M of retinoic acid and 10 ng/ml of ascorbic acid are performed with replacement of solution every three days.

EFFECT: invention makes it possible to obtain cardiomyicytal matrix suitable for direct transplantation.

7 dwg, 1 ex