FIELD: chemistry.
SUBSTANCE: method involves conducting a conjugation reaction by mixing ciprofloxacin and carbodiimide in ratio of 1:1 for 5 minutes at room temperature, adding the reaction mixture obtained as a result of conjugation to a solution of a human alpha-fetoprotein fragment followed by incubation of the obtained solution for 20-30 minutes while stirring and monitoring pH. By-products obtained from adding the reaction mixture to the solution of the human alpha-fetoprotein fragment are removed via dialysis against a phosphate-salt buffer at pH 8.0.
EFFECT: invention widens the field of using methods of producing conjugates of human alpha-fetoprotein with ciprofloxacin.
1 dwg, 1 ex
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