METHOD FOR PRODUCING SANDWICH-TYPE ISOLATED HEPATOCYTE CULTURE Russian patent published in 2011 - IPC C12N5/00 A01N1/02 

FIELD: medicine.

SUBSTANCE: method provides the following stages: (a) preparing a matrix, (b) preparing isolated hepatocytes, (c) inoculating a matrix of density 2 to 4x10³ mm^-2 with hepatocytes, (d) leaving the cell-coated matrix for 10-180 minutes so that to enable the cells to adhere to the matrix, (e) washing the nonadherent cells from the cell-coated matrix, (f) leaving the cell-coated matrix for max. 180 minutes, and (g) freezing the cell-coated matrix in a freezing medium, (h) storing the frozen cell-coated matrix, (i) defrosting the frozen cell-coated matrix, (j) washing the nonadherent cells from the cell-coated matrix, (k) coating the defrozen cell-coated matrix with a layer of a second matrix, and (1) reculturing the cells built-in between the matrixes.

EFFECT: invention allows providing an increased percentage of viable hepatocytes in the sandwich-type cultures.

12 cl, 4 dwg, 1 tbl, 4 ex