FIELD: medicine.
SUBSTANCE: what is disclosed is a method of determination of haplotypic polymorphism of an autosomic DNA by simultaneous amplification of a minisatellite marker and a closely spaced point substitute. The method consists in the fact that a specific version of an individual D1S 80 locus is amplified together with an allelic version located on the same chromosome of a single nucleotide polymorphic rs16824398 site with the use of an allel-specific polymerase chain reaction (PCR) with two primer versions with their 5'-end being marked with an appropriate fluorescent dye. A colour combination of the dyes of the allele-specific primer and a size of the allelic version of D1S 80 locus shows specific haplotypes inherent in an examined individual.
EFFECT: invention allows immediate determination of the specific haplotypes representing combinations of VNTR alleles of D1S80 locus and SNP rs16824398 site found on both chromosomes of the examined individual.
4 dwg, 1 tbl, 7 ex
