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2 830 245

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IPC

C12Q1/6806(2018-01-01)

C12Q1/6827(2018-01-01)

C12Q1/686(2018-01-01)

(21) (22)

Application

2024115503, 2024-06-06

(24)

Start date

2024-06-06

(22)

Actual filing date

2024-06-06

(45)

Published

2024-11-18

(72)

Inventor

Bushueva Olga Iurevna

(73)

Holder

Federalnoe Gosudarstvennoe Biudzhetnoe Obrazovatelnoe Uchrezhdenie Vysshego Obrazovaniia Gosudarstvennyi Meditsinskii Universitet" Ministerstva Zdravookhraneniia Rossiiskoi Federatsii

METHOD FOR GENOTYPING POLYMORPHIC LOCUS rs6457452 (C>T) OF HSPA1B GENE IN HUMAN BY REAL-TIME PCR USING ALLELE-SPECIFIC FLUORESCENT PROBES Russian patent published in 2024 - IPC C12Q1/6806 C12Q1/6827 C12Q1/686

Abstract RU 2830245 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, particularly to a method for genotyping a polymorphic locus rs6457452 (C>T) of the HSPA1B gene in humans. Method is carried out by means of a real-time PCR method and involves the use of forward and reverse primers with sequences of SEQ ID NO: 1 and 2 respectively, as well as rs6457452-C- and T-allele-specific fluorescent-labelled probes with sequences SEQ ID NO: 3 and 4 respectively.

EFFECT: present invention provides an accessible and easy-to-implement method of identifying single-nucleotide polymorphism rs6457452 (C>T) of the HSPA1B gene.

1 cl, 1 dwg

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RU 2 830 245 C1

Authors

Bushueva Olga Iurevna

Dates

2024-11-18—Published

2024-06-06—Filed