FIELD: genetic engineering, biotechnology, molecular biology. SUBSTANCE: invention can be used in medico-biological industry. Invention proposes the constructed recombinant plasmid (pACYCLANS) comprising the natural sequence of gene encoding L-asparaginase in Erwinia carotovora (ECAR-LANS) that is under control of promoter and terminator of phage T7 RNA-polymerase and encodes the full-scale enzyme precursor with signal peptide for export of protein to periplam followed by processing. Strain- producers with content of recombinant L-asparaginase 8-12% are prepared by transformation of strains E. coli with indicated plasmid that are able to synthesize phage T7 RNA-polymerase. For isolation and purification of enzyme from biomass method involves disruption of cells by ultrasonic disintegration, fractionation with ammonium sulfate and chromatography on column with SP-Sepharose FF. Invention provides preparing the purified L-asparaginase preparation with high yield. EFFECT: improved preparing method. 2 cl, 3 dwg, 1 tbl
| Title |
Year |
Author |
Number |
| GENE ENCODING L-ASPARAGINASE IN ERWINIA CAROTOVORA AND STRAIN ESCHERICHIA COLI VKPM = B-8174 AS PRODUCER OF ERWINIA CARATOVORA L-ASPARAGINASE |
2001 |
- Ehl'Darov M.A.
- Zhgun A.A.
- Gervaziev Ju.V.
- Aleksandrova S.S.
- Omel'Janjuk N.M.
- Archakov A.A.
- Skrjabin K.G.
- Sokolov N.N.
|
RU2221868C2 |
| RECOMBINANT PLASMID DNA pACYC-LANS(KM), STRAINS OF Escherichia coli BL21(DE3), TRANSFORMED RECOMBINANT DNA pACYC-LANS(KM) AND METHOD FOR PRODUCING A SUBSTANCE OF THE RECOMBINANT L-ASPARAGINASE FROM Erwinia carotovora |
2010 |
- Sidoruk Konstantin Vasil'Evich
- Bogush Vladimir Grigor'Evich
- Ehl'Darov Mikhail Anatol'Evich
- Goncharova Ol'Ga Vladimirovna
- Chugunova Nadezhda Mikhajlovna
- Pokrovskaja Marina Vladimirovna
- Aleksandrova Svetlana Serebedzhanovna
- Omel'Janjuk Natal'Ja Mikhajlovna
- Sokolov Nikolaj Nikolaevich
|
RU2441916C1 |
| RECOMBINANT DNA ENCODING FUNCTIONALLY ACTIVE HYBRID PROTEIN OF D-AMINO ACID OXYDASE WITH CHITIN-COUPLING DOMAIN (DAOcbd), pVR1 RECOMBINANT PLASMID PROVIDING SYNTHESIS THEREOF IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C 41 (DE3)/pVR1 AS PRODUCER OF DAOcbd |
2006 |
- Redo Vadim Aleksandrovich
- Ehl'Darov Mikhail Anatol'Evich
- Khatuntseva Svetlana Anatol'Evna
- Zhgun Aleksandr Aleksandrovich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2310688C1 |
| MODIFIED RAS GENE OF ESCHERICHIA COLI BACTERIUM, CODING PRECURSOR OF ENZYME WITH PENICILLIN G ACYLASE ACTIVITY, RECOMBINANT ESCHERICHIA COLI STRAIN - PRODUCER OF ACYLASE PENICILLIN G AND METHOD FOR MICROBIOLOGICAL SYNTHESIS OF THIS ENZYME |
2015 |
- Eldarov Mikhail Anatolevich
- Sklyarenko Anna Vladimirovna
- Dumina Mariya Vladimirovna
- Satarova Dzhenni Ernstovna
- Zhgun Aleksandr Aleksandrovich
- Medvedeva Natalya Viktorovna
- Yarotskij Sergej Viktorovich
|
RU2624022C1 |
| pETTvDAO2 RECOMBINANT PLASMID PROVIDING SYNTHESIS OF YEAST Trigonopsis variabilis D-AMINO ACID OXYDASE (DAO) IN Escherichia coli CELLS AND RECOMBINANT Escherichia coli STRAIN C41(DE3)/pETTvDAO2 AS PRODUCER OF DAO |
2006 |
- Ehl'Darov Mikhail Anatol'Evich
- Redo Vadim Aleksandrovich
- Zhgun Aleksandr Aleksandrovich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2310687C1 |
| METHOD FOR PRODUCING A SUBSTANCE OF THE RECOMBINANT L-ASPARAGINASE FROM ERWINIA CAROTOVORA |
2010 |
- Karasev Viktor Semenovich
- Bochkova Ol'Ga Petrovna
- Chugunov Aleksandr Mikhajlovich
- Melik-Nubarov Nikolaj Sergeevich
- Grozdova Irina Dmitrievna
- Chernovskaja Tat'Jana Veniaminovna
- Denisov Lev Aleksandrovich
- Rudenko Elena Georgievna
- Morozova Elena Leonidovna
- Bogush Vladimir Grigor'Evich
- Sidoruk Konstantin Vasil'Evich
- Koltun Igor' Olegovich
- Skatova Galina Evgen'Evna
- Abakumova Ol'Ga Jur'Evna
- Podobed Ol'Ga Vladimirovna
- Sokolov Nikolaj Nikolaevich
|
RU2441914C1 |
| RECOMBINANT PLASMID pSVH0106 PROVIDING SYNTHESIS OF Gl7ACA ACYLASE IN Escherichia coli CELLS, RECOMBINANT STRAIN OF Escherichia coli BL21(DE3)/pSVH0106 AS PRODUCER OF Gl7ACA ACYLASE |
2005 |
- Khatuntseva Svetlana Anatol'Evna
- Ehl'Darov Mikhail Anatol'Evich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2300566C2 |
| RECOMBINANT DNA, WHICH CODES FUNCTIONALLY ACTIVE HYBRID PROTEIN GL7ACA-ACYLASE WITH CHITIN-BINDING DOMAIN (BrdGL7ACA-cbd) AND RECOMBINANT PLASMID pSVH0108, PROVIDING FOR ITS SYNTHESIS IN CELLS Escherichia coli, RECOMBINANT STRAIN Escherichia coli BL21(DE3)/pSVH0108-PRODUCER BrdGL7ACA-cbd |
2006 |
- Khatuntseva Svetlana Anatol'Evna
- Ehl'Darov Mikhail Anatol'Evich
- Zejnalov Orkhan Akhmed Ogly
- Skrjabin Konstantin Georgievich
|
RU2388826C2 |
| MODIFIED TSA GENE ENCODING THE THERMOSTABLE L-ASPARAGINASE FROM THERMOCOCCUS SIBIRICUS ARCHAEA, RECOMBINANT ESCHERICHIA COLI STRAIN - THERMOSTABLE L-ASPARAGINASE PRODUCER AND METHOD FOR PURIFICATION OF THIS ENZYME |
2022 |
- Dumina Mariia Vladimirovna
- Zhgun Aleksandr Aleksandrovich
- Aleksandrova Svetlana Serebedzhanovna
- Pokrovskaia Marina Vladimirovna
- Sokolov Nikolai Nikolaevich
- Zhdanov Dmitrii Dmitrievich
|
RU2794225C1 |
| RECOMBINANT PLASMID DNA PTLS CONTAINING GENE OF SINGLE-STRANDED ANTIBODY AGAINST TICK-BORNE ENCEPHALITIS VIRUS AND STRAIN OF BACTERIUM ESCHERICHIA COLI AS PRODUCER OF SINGLE-STRANDED ANTIBODIES AGAINST TICK-BORNE ENCEPHALITIS VIRUS |
1998 |
- Nikolenko G.N.
- Tikunova N.V.
- Golovin S.Ja.
- Protopopova E.V.
- Loktev V.B.
- Il'Ichev A.A.
- Deev S.M.
|
RU2158309C2 |
