OLIGONUCLEOTIDE SETS FOR QUANTITATIVE DETERMINATION OF THE BOVINE LEUKEMIA PROVIRUS Russian patent published in 2021 - IPC C12Q1/68

Abstract RU 2747192 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to the field of biotechnology. The invention is a set of oligonucleotides for the analysis of bovine leukemia provirus by real-time polymerase chain reaction, it includes three forward primers: env_5400_F ttc-cct-ctg-tca-gat-cat-gg; env_5442_F cac-ggg-cct-tcc-cag-a; env F aag-ggc-ggc-gcc-ggt-tt, one reverse primer env_5620_R gga-agg-gtg-gtg-cca-tc and two TagMan fluorescent degradable probes: env_5441 (ROX) ct-acg-ggc-ctt-ccc -ag (RTQ2); env_5549 (FAM) ct-ccc-gga-cgc-cca-aa (RTQ1), which allows the quantitative determination of the BLV provirus with increased sensitivity and high specificity.

EFFECT: proposed set of oligonucleotides increases sensitivity, specificity and accuracy of the quantitative determination of the BLV provirus by RT-PCR.

1 cl, 3 dwg, 3 tbl

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RU 2 747 192 C1

Authors

Nam Irina Yan Gukovna

Akhmedov Roman Bakhtiyarovich

Zayakin Vladimir Vasilevich

Dates

2021-04-29—Published

2020-07-07—Filed