METHOD AND DEVICE FOR DELIVERY OF REDUCED PRESSURE TO CELL CULTURE Russian patent published in 2012 - IPC C12M1/12 

FIELD: food industry.

SUBSTANCE: cell cultivation method involves usage of a basically air-proof chamber, placement of cell matrix inside the said chamber, delivery of reduced pressure into the said chamber and delivery of cultural medium into the said chamber. The sealing device of the device for delivery of negative pressure to the cell matrix contains a cell culture container that includes a body with an outer wall and an inner volume, limited with a permeable membrane at one butt end and an open butt end positioned opposite the said membrane (the cultural medium penetrating through the permeable membrane to the cell matrix positioned inside the said container), a porous binder (placed onto the cell matrix so that the cultural medium penetrates through the cell matrix to the said binder), a drape (placed across the said open butt end of the cell culture container and forming sealing along the inner wall of the said container and the binder), the first tube (that penetrates through the said draper into the binder and flowing communicates with components on the both sides of the draper so that the cell matrix is subject to the effect of negative pressure through the said tube). A version of the device is envisaged.

EFFECT: invention usage will enable delivery of reduced pressure to cell cultures.

22 cl, 24 dwg