FIELD: chemistry.
SUBSTANCE: described are methods of producing simvastatin. In one version, the method is realised by reacting monocalin J and acyl thioester, which donates an acyl group to the C8 hydroxyl group of monacolin J in the presence of LovD acyltransferase; and LovD acyltransferase and regioselective acylation of the C8 hydroxyl group of monocalin J with LovD acyltransferase using the acyl group of acyl thioester. In the second version, the method is realised by reacting lovastatin and acyl thioester, which donates an acyl group to the C8 hydroxyl group of monacolin J in the presence of LovD acyltransferase and LovD acyltransferase and effect of LovD acyltransferase which leads to hydrolysis of lovastatin to monocalin J and using the acyl group of acyl thioester for regioselective acylation of the C8 hydroxyl group of monocalin J.
EFFECT: invention widens the range of the means of producing simvastatin.
12 cl, 24 dwg, 5 tbl, 4 ex
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