FIELD: chemistry.
SUBSTANCE: method involves passing a solution of lipopolysaccharide Shigella flexneri 2a in a 0.05 M triethylammonium acetate buffer at pH 4.7, which contains 10% n-propanol, through a column with a hydrophobic carrier which is Octyl Sepharose CL-4B, and then washing the column with the same buffer solution.
EFFECT: method can be used to produce carriers which enable extraction and purification of specific antibodies to lipopolysaccharides using affinity chromatography.
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