PROCESS OF FEED BATCH FERMENTATION AT HIGH CELL DENSITY FOR RECOMBINANT PROTEIN PRODUCTION Russian patent published in 2012 - IPC C12N1/20 C12P21/00 C12N15/09 

FIELD: food industry.

SUBSTANCE: described are version methods for production of recombinant meningococcal Proteins-2086 by way of feed batch fermentation with a bacterial culture with continuous supply of an inducer in a total quantity from approximately 7 g/l to approximately 15 g/l after the threshold parameter has been reached with continuous supply of a carbon source such as supply at a constant rate with subsequent meningococcal protein extraction from the bacterial culture. The cultivation method may be implemented using a bacterial host cell containing an expression vector encoding recombinant protein under control of an induced promoter or by means of a nucleic acid sequence with continuous supply of a carbon source (such as glucose) and an inducer (such as arabinose). Described are compositions for meningococcal protein extraction from the bacterial culture, produced by the said methods and noted for high Protein-2086 density. The protein may be lipidised or non-lipidised.

EFFECT: meningococcal Proteins-2086 yield improvement.

79 cl, 20 dwg, 7 tbl, 3 ex