FIELD: medicine.
SUBSTANCE: method involves nucleic acid recovery from analysed samples. Related oligonucleotide probes and primers are sampled, and PCR-amplification of target nucleic acid follows. Probe melting curves are analysed. Amplification of target nucleic acid is ensured by a relevant pair of primers, one of which, namely reverse, serves to form a DNA chain, complementary to the probe, and contains a non-fluorescent fluorescence quencher inside a nucleotide sequence, is closer to the 3' terminal. Detecting a site of target nucleic acid found between binding sites of primers is ensured by using specified 3'-fluorescent marked oligonucleotide probe a binding site of which is partially blocked with a determinant of an unmarked forward primer on one side, and on the other side it is spaced at a number of nucleotides from the 3'-terminal of the primer carrying the fluorescence quencher.
EFFECT: invention provides more effective real-time PCR analysis of specific nucleotide sequences and nucleotide replacements.
12 cl, 10 dwg, 3 tbl, 3 ex
