METHOD FOR DETECTING MUTATIONS IN THE parC AND gyrA QRDR GENE REGIONS LEADING TO RESISTANCE IN MYCOPLASMA GENITALIUM TO FLUOROQUINOLONE ANTIBIOTICS Russian patent published in 2022 - IPC G01N33/58 C12Q1/6806 C12Q1/6827 C12Q1/686 C12Q1/6876 

FIELD: medicine.

SUBSTANCE: invention relates to the field of medicine and biology. PCR amplification of the QRDR region of the parC and gyrA M. genitalium gene is carried out. The target sequence is detected with FAM and R6G fluorophores. The melting temperatures (Tm) of the obtained peaks are compared with the control samples and a conclusion is made about the presence of a mutation in the test sample. If the test sample has Tm=57°C±0.5°C on the FAM channel, this indicates the presence of a wild phenotype. If Tm=45-49°C, this indicates the presence of a mutation in parC QRDR. If the test sample has Tm =63-64°C on the R6G channel, this indicates the presence of a wild phenotype. If Tm=57-59°C, this indicates the presence of a mutation in gyrA QRDR.

EFFECT: invention provides detection of the presence of all possible mutations in the parC and gyrA genes of M. genitalium directly in the patient’s clinical material to select adequate antibiotic therapy and control the epidemiological process.

1 cl, 2 dwg, 3 tbl, 2 ex