FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology and concerns a strain of foot-and-mouth disease Aphtae epizooticae genotype O/CATHAY, family Picornaviridae, genus Aphthovirus, deposited in the Russian National Collection of Exotic types of foot-and-mouth disease virus and other animal pathogens FGBI "ARRIAH" under registration number: strain VYa O No. 2222/Taiwan/1/2012 (production and control porcine). Presented strain is reproduced in transplantable cell cultures of Siberian ibex kidney (PSGK-30), porcine kidney (IB-RS-2), newborn Syrian hamster kidney (BHK-21). In transplantable suspension cell culture of Syrian hamster kidney BHK-21/SUSP/ARRIAH for 11.65±0.41 hours of incubation, concentration of 146S component of the strain "O No. 2222/Taiwan/1/2012" of the foot-and-mouth disease virus has average values of 2.50±0.07 mcg/cm3 (75.09±0.42 %), preserving initial characteristics when passaging in a continuous suspension cell line BHK-21/SUSP/ARRIAH.
EFFECT: presented strain "O No 2222/Taiwan/1/2012" can be used for production of biopreparations for diagnosis and specific prevention of foot-and-mouth disease of genotype O/CATHAY and for controlling antigenic activity of FMD vaccines.
1 cl, 2 dwg, 5 tbl, 8 ex
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Authors
Dates
2024-04-09—Published
2023-09-04—Filed