FIELD: medicine.
SUBSTANCE: method involves the PCR-amplification of the 16S rRNA fragment with the use of specific original oligonucleotide primers SEQ ID No: 1 and SEQ ID NO: 2. It is followed by the PCR biotinylation of the primer SEQ ID No: 1 which enables detecting the nucleotide sequences of the 16S rRNA by simple one-chain solid-phase sequencing with the use of specific original sequencing primers SEQ ID No: 3, SEQ ID NO: 4 and SEQ ID NO: 5. The mycobacteria are precisely identified by comparing certain sequences with common sequences of various types of mycobacteria in the database.
EFFECT: invention enables identifying mycobacterioses in the samples of pathological material.
4 dwg, 1 tbl, 2 ex
