FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to a test system for detecting in a biomaterial DNA of mycobacteriosis agents included in Mycobacterium avium complex without differentiation to a species by real-time PCR. What is presented is a combination of primers specific to sequence of 16S-23S intergenic spacer and a fluorescent-labelled probe for detecting Mycobacterium species. Also disclosed is a kit of reagents comprising said combination and a PCR reaction mixture. Invention also relates to a method of detecting Mycobacterium species.
EFFECT: invention can be used to create medical devices for in vitro diagnostics, in order to detect the causative agents of mycobacteriosis by clinical and laboratory diagnostics services in medical institutions, as well as for the purpose of epidemiological surveillance of the population for the spread of mycobacteriosis agents.
5 cl, 1 dwg, 3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| SET OF HIGHLY SPECIFIC OLIGONUCLEOTIDE PRIMERS AND PROBES FOR THE DETECTION AND DIFFERENTIATION OF NON-TUBERCULOSIS MYCOBACTERIA | 2021 |
|
RU2765829C1 |
| DIAGNOSTIC METHOD AND A KIT FOR SIMULTANEOUS DETECTION AND IDENTIFICATION OF TUBERCULOSIS MYCOBACTERIUM AND NON-TUBERCULOSIS MYCOBACTERIA AND DETERMINATION OF RIFAMPICIN RESISTANCE OF TUBERCULOUS MYCOBACTERIUM ON THE BASIS OF QUANTAMATRIX ANALYTICAL PLATFORM | 2017 |
|
RU2715591C1 |
| SIMULTANEOUS DETERMINATION, IDENTIFICATION AND DIFFERENTIATION OF EUBACTERIAL TAXONS USING HYBRIDIZATION ANALYSIS | 1995 |
|
RU2154106C2 |
| SYSTEMS FOR TREATMENT OF PULMONARY INFECTIONS | 2013 |
|
RU2657510C2 |
| METHOD FOR PREPARING HIGH-BIOAVAILABILITY RIFABUTIN COMPOSITION, PHARMACEUTICAL COMPOSITION AND METHOD OF TREATING MYCOBACTERIOSIS | 2013 |
|
RU2520603C1 |
| METHOD OF IDENTIFYING MYCOBACTERIA BY POLYMERASE CHAIN REACTION | 2010 |
|
RU2455364C2 |
| COMPOSITIONS AND METHODS FOR DETECTION AND IDENTIFICATION OF NUCLEIC ACID SEQUENCES IN BIOLOGICAL SAMPLES | 2012 |
|
RU2595421C2 |
| METHOD OF DIAGNOSING TUBERCULOSIS | 1999 |
|
RU2163022C1 |
| METHOD FOR INCREASE OF REAL TIME POLYMERASE CHAIN REACTION SENSITIVITY IN DETECTION OF PATHOGENIC BACTERIA DNA | 2016 |
|
RU2636458C1 |
| OLIGONUCLEOTIDE PRIMERS AND METHOD OF DETECTING DNA OF Mycobacterium paratuberculosis THAT IS PARATUBERCULOSIS AGENT BY METHOD OF POLYMERASE CHAIN REACTION (PCR) | 2010 |
|
RU2435852C1 |
