FIELD: medicine.
SUBSTANCE: method for stabilising tularemia vaccine strain consists in introducing a gene recA deletion into a chromosomal DNA F, tularensis 15/10 with a suicide plasmid pPYΔrecA as a result of the allelic exchange of the modified DNA fragment with the deleted gene recA and a homologous region of the chromosomal DNA of the vaccine strain.
EFFECT: invention enables avoiding reversion of the vaccine properties and promotes the consistency of a genome of the vaccine tularemia microbial strain.
2 cl, 2 dwg, 6 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR ATTENUATION OF TULAREMIA VACCINE STRAIN | 2011 |
|
RU2460791C1 |
| Francisella tularensis 15/23-1ΔrecA STRAIN HAVING REDUCED REACTOGENICITY FOR PRODUCING LIVE TULAREMIA VACCINE AND METHOD FOR PRODUCTION THEREOF | 2014 |
|
RU2567810C2 |
| METHOD FOR DIFFERENTIATION OF TULAREMIA MICROBIAL SUBSPECIES | 2011 |
|
RU2478717C1 |
| RECOMBINANT PLASMID DNA ENCODING TUL4spCBD RECOMBINANT PROTEIN SYNTHESIS, M15[pREP4, pTUL4spCBD] ESCHERICHIA COLI STRAIN AS PRODUCER OF TUL4spCBD RECOMBINANT PROTEIN, TUL4spCBD RECOMBINANT PROTEIN, METHOD FOR PRODUCTION THEREOF, AND METHOD FOR PRODUCTION OF SPECIFIC ANTIBODY AGAINST TUL4spCBD PROTEIN | 2004 |
|
RU2270249C1 |
| METHOD OF OBTAINING BACTERIAL PRODUCER OF RECOMBINANT NC NUCLEOCAPPSID PROTEIN OF SARS-CoV-2 VIRUS | 2023 |
|
RU2812347C1 |
| METHOD OF OBTAINIG AN ATTENUATED PLASMID-FREE STRAIN OF F.TULARENSIS 15 CMSA, SYNTHESIZING MYCOBACTERIAL ANTIGEN SUPEROXIDE DISMUTASE A | 2019 |
|
RU2745161C1 |
| METHOD FOR THE DETERMINATION OF FRANCISELLA TULARENSIS SUBSPECIES BY MULTI-PRIMER PCR | 2021 |
|
RU2765495C1 |
| METHOD OF CHROMOSOMAL INTEGRATION AND REPLACEMENT OF DNA SEQUENCE IN CLOSTRIDIA | 2006 |
|
RU2464317C2 |
| ATTENUATED BACTERIA BORDETELLA PERTUSSIS, WHOOPING COUGH AGENT VACCINE | 2010 |
|
RU2455024C1 |
| PLASMID PXL 1635 | 1991 |
|
RU2127312C1 |
